Open wallac folder to see all available factory set protocols. Molecular sensors of estrogen receptor conformations and. Absorbance was read on a victor v multilabel plate reader perkinelmer, gaithersburg, md at a wavelength of a595 and data collected using wallac 1420 workstation software. Wallac 1420 workstation perkinelmer life sciences software. We use cookies to ensure that we give you the best experience on our website. Wallac 1420 multilabel counter is a complete platform for. The substrate solution was added, and the stop solution was added to each well after 25 minutes. Nov 12, 20 absorbance, or overall plate functional activity, was measured at 590 nm every 12 h up to 120 h or until the average plate absorbance reached 0. Nitrite assay in conditioned media because no is labile, its production could not be measured directly but was assessed from the concentration of nitrite in the medium. The luminometer expresses luminescence as counts per second cps.
Postoperative cognitive changes after total knee arthroplast. When wallac 1420 workstation is connected to an instrument running in normal mode, the demo mode indicator is blank. The optical density values were converted to digital data with the wallac 1420 workstation software perkin elmer. Perkin elmer wallac 1420 victor2 microplate reader gmi trusted. Operates as a standalone reader or with inputoutput. H 2 o 2 was used as a standard for intracellular ros production. Nitrite assay in conditioned media because no is labile, its production could not be measured directly but was assessed from. Perkin elmer wallac 1420 victor2 microplate reader gmi.
Measures fluorescence, luminescence, and absorbance. Fluorescence measurements were taken using wallac 1420 workstation software version 2. Acute respiratory distress syndrome neutrophils have a. Antibacterial metabolites from australian macrofungi from the. The samples were measured on a wallac victor 1420 multilabel counter using wallac 1420 workstation software version 2 wallac oy, turku, finland.
After a successful login wallac 1420 can be used according to the permissions granted to the logged on user. The wallac 1420 victor 2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. The most popular version of this product among our users is 1. Used wallac victor 2 1420 microplate reader for sale dotmed. Plates were read at 450 nm using the wallac victor3 1420 multilabel counter perkinelmer and wallac 1420 workstation software. This versatile instrument is designed for multitasking, and its ability to perform counting measurements in more than ten different modes on the same protocol makes it ideal for any nonisotopic assay. All of these have a lock icon, so they cannot be edited. Myd88 dependence of berylliuminduced dendritic cell. Molecular sensors of estrogen receptor conformations and dynamics. May 05, 2009 plates were read by using a victor 3 plate reader perkinelmer and the data analyzed by using wallac 1420 workstation software. Ease of use and general reliability are enhanced by 32bit windows workstation software, designed to utilize the full.
Users can take advantage of multiple assay technologies fitted in the wallac 1420 victor2 microplate reader. Austin weigle graduate research assistant university of. Development of a highthroughput method to evaluate serum. Victor x2 provides luminescence and fluorescence intensity fi detection technologies. The wallac 1420 victor 2 is a multilabel, an extended version of the successful wallac victor multilabel reader, wallac 1420 manual wallac perkin elmer 1420041 victor2 v plate reader perkin elmer. This instrument is designed to answer the strict requirements of biotechnology, industrial and academic laboratories. Explorer software to createedit protocols is accessed within the workstation software. Perkin elmer 1420 victor 3vincludes computer, software, looks greatavailable due to funding stopped at business. Energetics and surface properties of pseudomonas putida. Gained familiarity with plate readers and related software i. Differences between rlus were tested by the twotailed unpaired students ttest. Data analysis was performed using the wallac 1420 workstation software wallac 1420 manager, version 2. L were added to the required wells and the plates incubated for a further 4 h under the conditions. P2000 serverworkstation software installation manual.
The data analysis was performed using wallac 1420 workstation software wallac 1420 manager, version 2. Fluorescence experiments used a spex fluorolog ii model iiic cuvettebased fluorometer with data max 2. In vivo tumor studies all animal experiments were performed under protocols approved by the city of hope institutional animal care and use committee. Pdf small molecule inhibitors of apaf1related caspase 3. We report specific interactions between the transmembrane domains tmds of bax and the prosurvival bclxl bcell. The closer a result is to the upper limit of the range, the more red it will appear.
For fluorescence microscopic observation of intracellular ros production upon odorant stimulation in osn cultures, osns were loaded with 10. Victor x light provides luminescence detection technology. Fold induction was calculated as rlu in inoculated cells divided by rlu in noninoculated cells. Small molecule inhibitors of apaf1related caspase 39 activation that control mitochondrialdependent apoptosis article pdf available in cell death and differentiation 9. Preventive maintenance and repair on wallac liquid. The perkin elmer wallac 1420 victor2 is a high performing microplate reader built for multitasking functions. A blank well containing only dilution buffer was included in each plate and the background cps was subtracted from the samples cps values. The wallac 1420 victor 2 is a multilabel, an extended version of the successful wallac victor multilabel reader, wallac 1420 manual wallac perkin elmer 1420 041 victor2 v plate reader perkin elmer wallac 1420 victor 2 multilabel counter. Operating wallac 1420 19 when the workstation is used without an instrument i. You can switch between normal and demo modes using the options selection in the tools menu.
Refurbished perkin elmer wallac victor 2 multilabel counter. Bax transmembrane domain interacts with prosurvival bcl2. For each well, the average cps of three consecutive measurements per well was used, using the software wallac 1420 workstation. Perkinelmer life and analytical sciences, wallac oy, p. If you continue to use this site we will assume that you are happy with it. Twosided, homoscedastic t tests assessed significance. The wallac 1420 victor3 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. Atp binding and stabilization of the luminescent signal were performed according to manufacturers protocol promega following tumor cell lysis. Data were recorded by using a wallac 1420 workstation software. An extended version of the successful wallac victor multilabel reader, the victor3 allows immediate access to more than 10 counting modes. Absorbance at a wavelength of 450 nm were measured with a victor3 multilabel reader perkin elmer, waltham, ma. Right click on the link to the file and choose save target as and save the zip file to your desktop. Plates were read by using a victor 3 plate reader perkinelmer and the data analyzed by using wallac 1420 workstation software. The recruitment of the predominantly cytosolic bcl2 protein bax bcl2 associated x, apoptosis regulator to the mitochondria is associated with mitochondrial outer membrane permeabilization and apoptosis.
We own wallac 1420 multilabel counter user manual pdf, doc, epub, txt, djvu forms. Windows workstation software provides a userfriendly interface for defining protocols and integrating with other automated systems, making the victor 2 as convenient as it is flexible. Instrument manual perkinelmer 2030 multilabel reader 2030902001 march 2008 perkinelmer 2030 multilabel reader valid for instruments with software version 4. High stability and fast recovery of expression of the tol. Regulatory landscape of agerageoxidative stress axis and.
Dec 01, 2003 fluorescence experiments used a spex fluorolog ii model iiic cuvettebased fluorometer with data max 2. Wallac 1420 d workstation perkinelmer life sciences. Refurbished perkin elmerwallac victor 2 multilabel counter. The wallac 1420 victor2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single platform. Page 22 operating wallac 1420 you can choose the result range you want used to determine the colour code of the wells. The wallac 1420 instrument server can not be started. We will be glad if you go back to us again and again. Download wallac 1420 multilabel counter manual pdf user manual 1420 software version manual not relevant to the version of wallac 1420 you have.
The plate was analyzed using a victor 3 1420 multilabel plate reader with the wallac 1420 workstation software perkin elmer, waltham, ma with a 544. Perkin elmerwallac victor 2 multilabel counter iet. Small molecule inhibitors of apaf1related caspase 39. The photometer was equipped with two dispensers that were able to pump adjustable amounts of trisbuffer and luciferinluciferase solution into the wells of the microtiter plate, just. With powerful software and enhanced performance, victor x gives you the power of a bestinclass reader that works within your budget. Following 2 h incubation at 37 c, with orbital shaking 450 rpm, the absorbance of each well was recorded at 600 nm using a multiplate reader perkin elmer, victor 3 v with wallac 1420 workstation software. Bcl2 bcell lymphoma 2 proteins are key regulators of apoptosis. Oct 15, 2016 no nets are visible in the control samples. Wallac 1420 multilabel counter the wallac 1420 multilabel counter is a complete platform for quantitative detection of light emitting, or light absorbing markers. Copy left click whichever protocol you would like to use. A printed report is provided by each counter by means of printers controlled by the counters software.
Clicking the change password opens the change password dialogue where you can define a new password. Operating wallac 1420 18 type the appropriate useridpassword combination to proceed. The k d values were calculated using a two state model. Docetaxel taxotere cas 114977285 abmole bioscience. Calcium flux was monitored continuously, both before and after addition of agonist or drug indicated by the arrow, for a total of 612 minutes, at 535 and 660 nm, using a fluorescence plate reader and wallac 1420 workstation software wallac victor 2 1420 multilabel counter, perkin elmer. Used wallac victor 2 1420 microplate reader for sale.
Absorbance, timeresolved fluorescence trf, luminescence, fluorescence, fluorescence polarization. D the kinetics of net formation was assessed over 4 hours by measuring total fluorescence using a victor 3 multilabel reader using wallac 1420 workstation v3. Wallac 1420 workstation software, perkin elmer victor, tecan plate washers, as well as plate assay techniques. Use the links below to quickly find your software solution download. Bclxlinhibitory bh3 mimetics abt737 or abt263 and the. Evaluation of cell toxicity and flow cytometry analysis. Apr 22, 2015 fluorescence measurements were taken using wallac 1420 workstation software version 2. Surrogate indices of steatosis andor parameters of impaired fat metabolism aspartate aminotransferase ast, alanine transaminase alt, hepatic total lipids, hepatic triglycerides and hepatic total cholesterol were measured as previously described 34. The victor 3v model 1420041 is the topoftheline model in the victor series.
Perkin elmerwallac victor 3v multilabel microplate reader for. The wallac 1420 victor2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay technologies on a single. All animal experiments were performed under protocols approved by the city of hope institutional animal care and use committee. The victor 2 can accommodate plates in formats between 6 and 864 wells read more. Upstream open reading frames cause widespread reduction of. The wallac victor 2 is a high performance multilabel plate reader.
Depending on the exact model, it is suitable for flash or glow luminometry, fluorometry, highsensitivity timeresolved fluorometry. Regional delivery of chimeric antigen receptorengineered t. Absorbance, or overall plate functional activity, was measured at 590 nm every 12 h up to 120 h or until the average plate absorbance reached 0. An extended version of the successful wallac victor multilabel reader, the victor2 allows immediate access to more than 10 counting modes. Perkinelmer has led the way for over 25 years with powerful software and informatics solutions. The wallac model 1409001 liquid scintillation counter and wallac model 1480011 gamma counter are used to detect radioactive contamination in samples collected, prepared and counted on these two devices. Samples were measured on wallac victor 1420 multilabel counter using wallac 1420 workstation software version 2 wallac oy, turku, finland. Mar 11, 2015 the plate was analyzed using a victor 3 1420 multilabel plate reader with the wallac 1420 workstation software perkin elmer, waltham, ma with a 544nm excitation filter and a 600nm emission filter.
987 1036 62 1134 814 485 931 390 1235 506 1093 1069 927 354 663 115 798 1371 1125 82 866 974 865 526 698 1585 996 1357 868 865 144 148 891 794 474 381 776 159 1341 1486 291 823 1267